Dynamic Fluctuations in Subcellular Localization of the Hippo Pathway Effector Yorkie In Vivo

SA Manning; LG Dent; S Kondo; ZW Zhao; N Plachta; KF Harvey

Journal article

Dynamic Fluctuations in Subcellular Localization of the Hippo Pathway Effector Yorkie In Vivo

SA Manning, LG Dent, S Kondo, ZW Zhao, N Plachta, KF Harvey

Current Biology | Published : 2018

DOI: 10.1016/j.cub.2018.04.018

Abstract

The Hippo pathway is an evolutionarily conserved signaling network that integrates diverse cues to control organ size and cell fate. The central downstream pathway protein in Drosophila is the transcriptional co-activator Yorkie (YAP and TAZ in humans), which regulates gene expression with the Scalloped/TEA domain family member (TEAD) transcription factors [1–8]. A central regulatory step in the Hippo pathway is phosphorylation of Yorkie by the NDR family kinase Warts, which promotes Yorkie cytoplasmic localization by stimulating association with 14-3-3 proteins [9–12]. Numerous reports have purported a static model of Hippo signaling whereby, upon Hippo activation, Yorkie/YAP/TAZ become cyt..

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University of Melbourne Researchers

Kieran Harvey Author

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Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

We thank Nicolas Tapon for discussions, Yanina Alvarez for assistance with data analysis, and the Peter Mac Microscopy and Histology Core facility for assistance. We thank Jin Jiang, Iswar Hariharan, Duojia Pan, Nicolas Tapon, the Vienna Drosophila Resource Center, the Australian Drosophila Research Support Facility (http://www.ozdros.com), the Bloomington Drosophila Stock Center, and the Developmental Studies Hybridoma Bank for D. melanogaster stocks and antibodies. K.F.H. is a National Health and Medical Research Council Senior Research Fellow. S.A.M. was supported by an Australian Postgraduate Award. This research was supported by the National Health and Medical Research Council of Australia (1078220 and 1080131) and the CASS Foundation.